Dr Damian Cummings
I use single cell and field electrophysiology complemented with fluorescent immunohistochemistry, confocal imaging and behavioural approaches to understand the interactions of neurones and microglia to the development of early Alzheimer's disease pathologies. I am also involved with teaching in both the lab and classroom environments.
My project is focused on assesing the microglia influence in synaptic transmission and early amyloid beta plaque deposition in knock-in mice.
My PhD project is part of a collaboration with Jörg Hanrieder at the University of Gothenburg and involves looking at the precise dynamics of Aβ aggregation and plaque formation in APP knock-in mice and organotypic hippocampal slice cultures, using stable isotope labelling and MALDI imaging mass spectrometry.
My project aims to understand the early mechanisms of Alzheimer's disease and specifically, the role of microglia in mediating the early response of the brain to the pathological amyloid plaques and their effects on axonal transmission.
Since finishing my MSc at UCL last year, I have gained further research experience working as a research technician in our lab, working on a funded research project aiming at studying the relationship between gene expression and pathology in APPKI mice which have humanised APP with mutations that cause Alzheimer's disease in humans. We will make our data publicly available through our open database (http://www.mouseac.org/, Matarin et al., 2015). We hope to improve the understanding of gene expression profile in AD.
I am molecular biologist postdoc working on Alzheimer's disease pathology, using newly-developed APP KI mice models. My current focus is on developing plaques in organotypic slices from these mice to characterize early changes confirming the onset of pathology. I am also involved in supervising students in the lab.
I am investigating the functions of predicted risk genes for Alzheimer's disease in primary microglial cultures from trem2 KI mice. I am looking at how these genes behave in inflammatory and anti-inflammatory conditions. As well as how they could be interacting.
My project focuses on imaging and analysis of dendritic spines in the mouse hippocampus using confocal microscopy. I am interested in understanding the effects of diets (high sugar, high fat) on properties of dendritic spines while further investigating the relationship of diseases like obesity and diabetes with Alzheimer's disease.
I’m a student of MSc Neuroscience in UCL. I’m doing my master project in Edwards lab. My project is about characterising microglia activity in App knock-in mice during the early stage of beta-amyloid deposit in the hippocampus, including its association with plaques and differences between genders.
I’m working on the characterisation of predicted risk genes associated with Amyloid-beta pathology of Alzheimer’s disease. I assessed their gene and protein expression in microglia in response to plaque deposition, with altered function and expression of TREM2, and in inflammatory conditions.
I am studying the effect of high-fat and high-sugar diets on synaptic transmission by recording field potentials in the CA1 hippocampal region. In order to do so, I will investigate the basal synaptic transmission and long-term potentiation in hippocampal slices taken from mice in high-fat and high-sugar diets.
UCL Brain Bank
Ken Smith, RoshiDesai
Henrik Zetterberg (UCL)
Riken Japan (APPKI mice)